中国卒中杂志2012，Vol.7Issue(2)：103-108,6.
亚低温对大鼠脑缺血再灌注后出血转化及超微结构的影响
Effects of Subhypothermia on Hemorrhagic Transformation and Uitramicrostructure after Middle Cerebral Ischemia-Reperfusion
摘要
Abstract
Objective To investigate the effect of sub-hypothermia on hemorrhagic transformation and ultra-structure of the rats with cerebral ischemia-reperfusion within or beyond 3 hours-the therapeutic time window, and to study the neuroprotective effect of subhypothermia on cerebral ischemia in rats. Methods Using the intra-luminal suture method, we subjected Sprague-Dawley rats to middle cerebral artery occlusion(MCAO) model and reperfusion 3 hours, 6 hours or 9 hours later, and then the rats were divided into normal temperature group, subhypothermia for 3 hours group and subhypothermia 5 hours group. A group of sham-operated animals without ischemic was used as control. At the 24th hour after ischemia, all rats were killed. Six rats were selected in each group for measuring the amount of bleeding according to the method described by Sumii T et al. Two or 3 rats were selected in each group for observing morphologic changes of endothelial cell, gliacyte, neuron and neuropile in retrochiasmatic cortex ischemic boundary zones by electron microscopy. Results The cerebral injury and hemorrhagic transformation aggravated as the time prolonging after ischemia-reperfusion,subhypothermia should prevent the development of the injury and hemorrhagic transformation. Observation under transmission electron microscopes showed that cerebral ultrastructure of retrochiasmatic cortex ischemic boundary zones was damaged in different degrees in MCAOR groups, and the cerebral ultrastructure injury increased in cortex ischemic boundary zones as the ischemic time prolonging. Subhypothermia could alleviate the nerve cell damage. It showed the most effective in MCAO3hR+HT3h group. Conclusion Subhypothermia has protective effect on brain ultrastructure of rats with cerebral ischemia reperfusion injury. It provides ultrastructural foundation for brain functional reconstruction.关键词
脑缺血/再灌注/出血转化/超微结构/亚低温Key words
Cerebral, ischemia-reperfusion/ Hemorrhagic transformation/ Ultrastructure/ Subhypothermia引用本文复制引用
孔伟,吴硕琳,毛淑静,沈东超,张宁,王春雪,李菁晶..亚低温对大鼠脑缺血再灌注后出血转化及超微结构的影响[J].中国卒中杂志,2012,7(2):103-108,6.基金项目
北京市自然科学基金(7102050)北京市卫生系统高层次卫生技术人才培养计划(2011-3-023) （7102050）
