中华临床免疫和变态反应杂志2012，Vol.6Issue(1)：1-5,5.
肿瘤坏死因子-α基因启动子376位点基因多态性与强直性脊柱炎的相关性
Association of Gene Polymorphisms of Tumor Necrosis Factor-alpha at Position-376 with Ankylosing Spondylitis
摘要
Abstract
Objective To investigate the association between tumor necrosis factor-alpha ( TNF-α ) 376 gene mutation and ankylosing spondylitis( AS ) in Chinese Han population. Methods A correlation analysis was performed in a case-control cohort of 164 AS cases and 121 controls, two single nucleotide polymorphisms ( SNPs ) located in the promoters of TNF-a gene ( -376 G/A )were examined by allele specific amplification. Statistics product and service solutions( SPSS ) software programs was used to calculate genotype and alleles frequencies and was applied to test the relationship between frequencies of genotype and alleles and AS. Results TNF-α-376 genotypes were G/G homozygote in 89% , A/A homozygote in 1. 2% , and G/A heterozygote in 9. 8% respectively in AS patiens. While TNF-α-376 genotypes were G/G homozygote in 92. 6% , A/A homozygote in 0. 8% , and G/A heterozygote in 6. 6% respectively in the control group. No significant difference was found in the distribution of TNF-a-376 genotype between both groups ( χ2 = 1. 016, P = 0. 602 ). The frequencies of TNF-a-376 alleles in AS patients were 93. 9% for G and 6. 1 % for A, while they were 95. 9% for G and 4. 1% for A in the control group. No significant differences ( χ2 =0. 721, P = 0. 346 ) were found at the polymorphic alleles in the TNF-α-376 G/A genes. Conclusions AS is not associated with polymorphism of TNF-a-376 in Chinese Han population. TNF-a-376 gene may do not be involved in AS susceptibility.关键词
强直性脊柱炎/肿瘤坏死因子/基因型/基因突变Key words
spondylitis ankylosing/tumor necrosis factor-alpha/genotype/gene mutation分类
医药卫生引用本文复制引用
李志华,韩捷,马群,付嘉,李春霞,张惠,司传平..肿瘤坏死因子-α基因启动子376位点基因多态性与强直性脊柱炎的相关性[J].中华临床免疫和变态反应杂志,2012,6(1):1-5,5.基金项目
山东省济宁市科技局立项项目(编号:JK200948) （编号:JK200948）
