广东海洋大学学报2011，Vol.31Issue(6)：20-25,6.
勒氏笛鲷(Lutjanus russellii)肌肉生长抑制素基因的克隆与分析
Cloning and Analysis of the Myostatin (MSTN) Gene in Russell's Snapper, Lutjanus russellii
摘要
Abstract
Myostatin (MSTN) gene was isolated from Russell's snapper, Lutjanus russellii by homology cloning method with the genome DNA. The Russell's snapper MSTN was 2887 bp in length, including three exons and two introns, along with 101 bp 5-UTR (untranslated region), 385 bp exon 1, 354 bp intron 1, 370 bp exon 2, 761 bp intron 2, 381 bp exon 3 and 1 932 bp 3'-UTR. The Russell's snapper MSTN gene encodes a 378 amino acid protein, including nine conserved cysteine residues, a RVRR proteolytic cleavage domain, and a putative signal peptide of 22 amino acids. Amino acid sequence analyses show the Russell's snapper MSTN has closer affinity with type 1 MSTN than type 2 MSTN of several telests. And it has the highest identity with Perciformes, higher identity with Cypriniformes, and the least identity with that of mouse, human and chicken MSTN. Neighbor-joining method and the results also show Russell's snapper has the closest homology with morone. And it clusters with type 1 MSTN of fish. It suggests that the Russell's snapper MSTN we isolated may belong to type I MSTN.关键词
勒氏笛鲷/肌肉生长抑制素(MSTN)/基因/克隆/序列分析Key words
Lutjanus russellii/ Myostatin (MSTN)/ cloning/ sequence analysis分类
生物科学引用本文复制引用
邓思平,李嘉颖,张婉玲,宋春杰,李广丽,朱春华,吴天利..勒氏笛鲷(Lutjanus russellii)肌肉生长抑制素基因的克隆与分析[J].广东海洋大学学报,2011,31(6):20-25,6.基金项目
广东海洋大学博士启动项目(0712103) （0712103）
广西科学研究与技术开发计划重点项目(桂科合1140009-4) （桂科合1140009-4）
