广东海洋大学学报2011，Vol.31Issue(6)：26-31,6.
马氏珠母贝多聚泛素基因重复单元及3′端非编码区序列的克隆与表达分析
Cloning and Expression Analysis of Repeat Unit and 3′ Untranslated Region eDNA Sequences of Polyubiquitin from Pinctadafucata
摘要
Abstract
Based on the construction of a suppression subtraction hybridization (SSH) library from haemocyte, the repeat unit and 3' untranslated region (UTR) cDNA sequences of polyubiquitin from Pinctada fucata (designated PfUb) were cloned by rapid amplification of cDNA ends (RACE) technique. The sequence was 453 bp, consisting of a 3' UTR of 219 bp and an open reading frame (ORF) of 234 bp encoding a polypeptide of 77 amino acids. Similar to other polyubiquitin, PfUb contained 8 functional sites, including 6Lys, 11Lys, 27Lys, 29Lys, 33Lys, 48Lys, 63Lys and 76Gly in the repeat unit. Real-time PCR analyses indicated that PfUb mRNA was ubiquitously expressed in all tested tissues, and its expression was increased, especially in gill and haemocyte, after Vibrio alginolyticus challenge. The temporal expression of PfUb mRNA in haemocytes challenged by V. Alginolyticus was time-dependent. It began to function at 2 h post-challenge and reached the maximum level at 8 h post-challenge.关键词
马氏珠母贝/多聚泛素/基因/重复单元/非编码区/克隆/表达/RACE/RT-PCRKey words
Pinctada fucata/ polyubiquitin/ gene/ repeat unit/ untranslated region/ clone/ expression/ RACE/ RT-PCR分类
生物科学引用本文复制引用
王忠良,简纪常,鲁义善,吴灶和..马氏珠母贝多聚泛素基因重复单元及3′端非编码区序列的克隆与表达分析[J].广东海洋大学学报,2011,31(6):26-31,6.基金项目
国家星火计划"南海水产动物病害防控技术应用示范"(2008GA780013) （2008GA780013）
