浙江农林大学学报2012，Vol.29Issue(1)：58-64,7.
土壤中山核桃干腐病抑制菌的筛选和鉴定
Screening and identification of inhibiting strains for Carya cathayensis canker disease
摘要
Abstract
The objective of this paper was to get a few of available inhibiting strains against Carya cathayensis canker disease. Inhibition assay was used to preliminarily screen strains from soils that inhibited Botryosphaeria dothidea, the pathogen of Carya cathayensis canker disease. Then, phenotypic characteristics and analysis of internal transcribed spacer (ITS) fragments were used to identify the strains. Results of the preliminary screening revealed five strains. After being cultured against pathogen, two of five strains, QZ2 and QZ8, exhibited inhibiting capability with apparent inhibition zones between the pathogen and strains QZ2 and QZ8 being observed. Spores of strain QZ2 finally covered the pathogen completely with pathogen growth ceasing. QZ2, which controlled the canker disease by parasitizing the disease strain, was identified as Trichoderma asperel-lum. Strain QZ8, which inhibited disease pathogen growth by creating antibiotics, was identified as Penicilli-um oxalicum. It can be concluded that QZ2 and QZ8 are potential strains for development of bio-controller a-gainst Carya cathayensis canker disease.关键词
森林保护学/山核桃干腐病/抑制菌/筛选鉴定/ITS序列分析Key words
forest protection/ Carya cathayensis canker disease/ inhibiting strain/ screening and identification/ internal transcribed spacer (ITS) sequence analysis分类
农业科技引用本文复制引用
田甜,沈振明,徐秋芳,刘卜榕..土壤中山核桃干腐病抑制菌的筛选和鉴定[J].浙江农林大学学报,2012,29(1):58-64,7.基金项目
浙江省研究生创新科研基金资助项目(YK2008074) （YK2008074）
