浙江农林大学学报2012，Vol.29Issue(1)：130-136,7.
兴安落叶松基本群体与育种群体RAPD多样性分析
Diversity analysis with RAPD for natural and breeding populations of Larix gmelinii
摘要
Abstract
Random amplified polymorphic DNA (RAPD) markers associated with phenotypic characteristics were used to analyze the genetic variation of 270 individual samples from six natural populations of Larix gmelinii (Wuyiling, Ganhe, Kuduer, Nenjiang, Moerdaoga, and Aershan). From 450 primers, 23 clear RAPD primers were selected as their amplified bands with high polymorphism. The analysis included the gene diversi ty index, Shannon's diversity index (SI), Nei's gene diversity index, a cluster analysis, and a correlation analysis. Results showed 182 loci among a total of 186 were polymorphic or a percentage of polymorphism (PPB) of 97.9% having a range of 87.10%-94.09%. The gene diversity index was 0.374 3, and the genetic diversity within populations was 0.051 7 indicating that genetic variation was mainly within the population (86.19%). Mean SI was 0.4821, and the mean for Nei's index was 0.322 6. The cluster analysis revealed six populations divided into three groups (the Xiaoxing'an Mountain Range, northwest of the Daxing'an Mountain Range, and south of the Daxing'an Mountain Range) with a genetic distance of 0.09. Genetic distance was significantly and positively correlated with geographical distance (P<0.05, r=0.768). Growth traits of the four L. Gmelinii populations were 10.25%-20.78% for height, 14.93%-28.37% for diameter, and 35.03%-74.36% for volume coefficients of variation. Compared with the genetic variation of breeding populations, in natural populations PPB was 5.32% greater, mean of effective alleles numbered 1.13% greater, Nei's index was 4.94% greater, SI was 5.68% greater, and the populations' total genetic variation 8.78% greater.关键词
林木育种学/兴安落叶松/基本群体/育种群体/随机扩增多态性DNA/生长性状/遗传多样性Key words
forest tree breeding/ Larix gmelinii/ natural population/ breeding population/ RAPD/ growth traits/ genetic diversity分类
农业科技引用本文复制引用
张振,张含国,张磊,朱航勇,李雪峰..兴安落叶松基本群体与育种群体RAPD多样性分析[J].浙江农林大学学报,2012,29(1):130-136,7.基金项目
国家科技基础性工作专项(2007FY 110400-3) （2007FY 110400-3）
