浙江医学2012，Vol.34Issue(5)：336-338,341,4.
酶联免疫斑点法检测甲型H1N1流感患者Th1/Th2细胞的变化
Change of Th1/Th2 lymphocyte subsets in patients with influenza A(H1N1) infection and its significance
周仁芳 1曾爱平 1方合志 1何龙 1蔡丽敏2
作者信息
- 1. 317500,温州医学院附属温岭医院检验科
- 2. 317500,温州医学院附属温岭医院感染科
- 折叠
摘要
Abstract
Objective To investigate the change of Th1/Th2 lymphocyte subsets in patients with influenza A (H1N1) infectionand its clinical significance.Methods MethodsLymphocyte subsets were analyzed by enzyme-linked immunospot (ELISPOT) assay in40 patients with influenza A(H1N1) infection, 42 influenza-like patients and 45 healthy subjects.results ResultsWhite blood cell, lym-phocyte and T cell including T4 and T8 in H1N1 patients were significantly lower than those in influenza patients and healthy controls (P<0.05). B cell in H1N1 patients was also significantly lower than that in control subjects (P<0.05). There were no significance differences in NK cells among three groups(P >0.05). Median value of IFN-y specific spot forming cells (SFC) in H1 patients, influenza-like patients and healthy controls were 23, 36.5 and 43 respectively! P<0.05).Median value of IL-4 specific spot forming cells (SFC) in H1N1 patients was higher than that in controls (36.5 vs 23, P<0.05) , but there was no difference between H1N1 patients and influenza-like patients(36.5 vs 27, P >0.05).concluslon H,N, virus infection is associated with abnormality of lymphocyte amount and its subsets, which can be detected by ELISPOT technology at a single cell level.关键词
酶联免疫斑点法/甲型H1N1流感/Th1//Th2细胞/免疫功能Key words
Enzyme linked immunospot/Influenza A (H1N1)/Th1/Th2 cells/ Immune function引用本文复制引用
周仁芳,曾爱平,方合志,何龙,蔡丽敏..酶联免疫斑点法检测甲型H1N1流感患者Th1/Th2细胞的变化[J].浙江医学,2012,34(5):336-338,341,4.
