肿瘤防治研究2012，Vol.39Issue(2)：137-139,3.DOI:10.3971/j.issn.1000-8578.2012.02.005
Pokemon基因在肝癌细胞中的表达及意义
Pokemon Gene Expression in Hepatoma Cells and Its Significance
摘要
Abstract
Objective To research pokemon expression in hepatoma cells and apoptosis of liver cancer cells through down-regulation of Pokemon Gene. Methods Pokemon expression was detected by western blot assay in hepatoma cellular lines HepG2, SMMC7721 and human embryonic stem cells LO2 cell lines. Pokemon gene silencing was induced by siRNA inhibition and then apoptosis of hepatoma cells was analyzed by flow cytometry. Results Pokemon expressions in HepG2 and SMMC7721 were significantly higher than those in human fetal liver cells LO2. siRNA inhibition of the expression of Pokemon triggered apoptosis of the liver cancer cells. Conclusion Proto-oncogene Pokemon expression in liver cancer cells was significantly increased, and played an important role in hepatocellular carcinoma development.关键词
Pokemon基因/肝细胞癌/基因沉默Key words
Pokemon/ Hepatocellular carcinoma( HCC) /Gene silencing分类
医药卫生引用本文复制引用
赵心恺,宁巧明,孙晓宁,田德安..Pokemon基因在肝癌细胞中的表达及意义[J].肿瘤防治研究,2012,39(2):137-139,3.基金项目
海南省卫生厅科研立项资助课题(琼卫2010-8) （琼卫2010-8）
