肿瘤防治研究2012，Vol.39Issue(2)：150-153,4.DOI:10.3971/j.issn.1000-8578.2012.02.008
5-Aza-dC对胰腺癌细胞系Panc-1中TFPI-2基因甲基化水平及表达的影响
Effect of 5-Aza-dC on Expression and Methylation of TFPI-2 Gene in Panc-1 Pancreatic Cancer
摘要
Abstract
Objective To investigate the effects of 5-aza-2,-deoxycytidine(5-Aza-dC) 、a methylation inhibitor, on the expression and methylation of TFPI-2 gene in Panc-1 cell lines of pancreatic cancer. Methods Panc-1 cell line was treated with different dosages of 5-Aza-dC. TFPI-2 gene DNA.mRNA and protein were determined by MSP,RT-PCR and Western blot respectively. Results MSP detection showed that the TFPI-2 gene hypermethylation has effectively been reversed by 5-Aza-dC. Moreover, the expression levels of TFPI-2 mRNA treated with 5-Aza-dC were increased (0. 211 ± 0. 087,0. 327 ± 0. 068 and 0. 609 ± 0.017,respectively). Western blot indicated that 5-Aza-dC could recover the TFPI-2 protein expression (0. 429 ± 0. 121,0. 675 ± 0. 044 and 1. 132 ± 0. 124 respectively). These effects within certain extent were dose and time dependent with statistical significance (P<0. 05). Conclusion The hypermethylation of promoter region was a main cause for transcriptional inactivation of TFPI-2 gene in Panc-1 cell lines. 5-Aza-dC might effectively reactivate the gene transcription through a demethylation role.关键词
胰腺癌/Panc-1/TFPI-2基因/甲基化/5-氮杂-2'-脱氧胞苷Key words
Pancreatic cancer/Panc-l / TFPI-2 gene/Methylation/5-Aza-dC分类
医药卫生引用本文复制引用
郑浩,汤志刚..5-Aza-dC对胰腺癌细胞系Panc-1中TFPI-2基因甲基化水平及表达的影响[J].肿瘤防治研究,2012,39(2):150-153,4.基金项目
国家自然科学基金资助项目(81071734) （81071734）
