肿瘤防治研究2012，Vol.39Issue(2)：157-160,4.DOI:10.3971/j.issn.1000-8578.2012.02.010
国产雷帕霉素对人淋巴瘤细胞Raji增殖的影响
Effect of Rapamycin in Proliferation of Human Burkitt Lymphoma Cells
王炜 1王志彬 1高玉环2
作者信息
- 1. 071000 河北保定,保定市第一中心医院血液科
- 2. 河北医科大学第四医院暨河北省肿瘤医院血液科
- 折叠
摘要
Abstract
Objective To investigate rapamycin effects on growth inhibition and mTOR/p7()S6K signaling pathway of human Burkitt lymphoma cell line Raji cells. Methods Proliferations of Raji cells under different of concentrations (0,1,5.10.20,40,50 and 10()nmol/L) and different times (24,48 and 72 h) were investigated by MTT assay. Apoptosis and cell cycle were analyzed via flowcytometry. The morphological alterations were confirmed by the optical microscope. The expressions of mTOR,p7()S6K,p-p70S6K proteins were examined by Western blot technique in the rapamycin-treated and untreated Raji cells. Results Rapamycin inhibited the proliferation of Raji cells at concentrations more than 5nmol/L(P<0. 05),in a dose and time dependent manner. After treatment with rapamycin,the number of cells at S phase and G2/ M phase was decreased gradually (P<0. 05) .but significantly increased at G0/G1 phase in dose and time dependent manners (P<0. 05). However,evident apoptosis did not observed in Raji cells. The expression of mTOR,p-p70S6K proteins was decreased gradually while the expression of p70S6K protein was increased in a concentration- dependent manner (P<0. 05). Conclusion mTOR/p70S6K signaling pathway was constitutively activated in Raji cells and Rapamycin inhibited Raji cell proliferation by arrest at G0/G1 phase and inhibition of mTOR/p70S6K signaling pathway.关键词
国产雷帕霉素/Raji细胞/细胞增殖/mTOR/p-p70S6KKey words
Rapamycin/Raji cell/Proliferation/mTOR/p-p70S6K分类
医药卫生引用本文复制引用
王炜,王志彬,高玉环..国产雷帕霉素对人淋巴瘤细胞Raji增殖的影响[J].肿瘤防治研究,2012,39(2):157-160,4.
