中南大学学报（自然科学版）2012，Vol.43Issue(1)：1-7,7.
中等嗜热菌浸出黄铜矿的温度影响规律及种群组成分析
Temperature influence on chalcopyrite bioleaching by moderate thermophile bacteria and microbial population analysis
摘要
Abstract
Bioleaching of chalcopyrite with moderate thermophilic bacteria was conducted at different temperatures. The results show that the moderate thermophiles can present bioleaching ability at 12-55 °C during leaching process. The optimum growth temperature is 45-50 °C. When the temperature reaches 60 °C, moderate thermophiles can maintain activity in the initial stage, but after a few days the bacteria concentration reduces gradually. 16S rDNA clone library reveals that the composition of moderate thermophiles at 45 °C and 60 °C are mainly Leptospirillum ferriphilum, Acidithiobacillus caldus, Sulfobacillus thermotolerans and Acidosphaera rubrifaciens. Leptospirillum ferriphilum is the dominant bacteria at the both temperatures, and archaea Fermplasma cupricumulans are found at 60 °C, which can use organic substances produced by other bacteria as energy source. It is suggested that these bacteria can supplement each other during bioleaching, thus facilitating the dissolution of chalcopyrite.关键词
细菌浸出/黄铜矿/中等嗜热菌/克隆文库Key words
bioleaching/ chalcopyrite/ moderate thermophile/ clone library分类
矿业与冶金引用本文复制引用
温建康,徐金光,陈勃伟,武彪,姚国成,刘美林,王淀佐..中等嗜热菌浸出黄铜矿的温度影响规律及种群组成分析[J].中南大学学报（自然科学版）,2012,43(1):1-7,7.基金项目
国家高技术研究发展计划(“863”计划)项目(2007AA060900) （“863”计划）
国家自然科学基金重点资助项目(50934002) （50934002）
国家科技支撑计划项目(2006BAB02A08) （2006BAB02A08）
