中山大学学报（自然科学版）2012，Vol.51Issue(1)：76-81,6.
加州鲈诺卡菌病病原的分离与鉴定
Isolation and Identification of Nocardia, A Pathogen of Nocardiosis in Largemouth Bass, Micropterus salmoides
摘要
Abstract
Nocardiosis is a commercially important disease in cultured largemouth bass in China, causing substantial economic losses. However, the pathogen causing largemouth bass nocardiosis has not yet been isolated in China. In this study, a strain of Nocardia (designed as NH090627 ) was isolated from diseased largemouth bass reared in Nanhai, Guangdong. The bacteria appeared Gram-positive, bead-like or long, slender, filamentous rods in shapes. The traditional physiological and biochemical methods showed that most of the phenotype of strain NH090627 were similar to Nocardia. Phylogeny analysis with 16S rDNA showed that the strain NH090627 was branched into the same cluster as that of other species of Nocardia , and had a higher identity with Nocardia seriolae JCM 3360T (99. 9% ). Based on morphological, physiological, biochemical properties and phylogenetic analysis, the filamentous rod bacterial was identified as Nocardia seriolea. Antimicrobial susceptibility assay showed that this strain was susceptible to chloramphenicol, streptomycin, kanamycin and tetracycline, but high resistant to sulphamethoxazole and ampicillin.关键词
加州鲈/鰤鱼诺卡菌/生理生化特征/16S rDNA/药敏试验Key words
largemouth bass/ Nocardia seriolae/ physiological and biochemical properties/ 16S rDNA/ drug sensitivity test分类
农业科技引用本文复制引用
蒋依依,李言伟,周素明,李安兴..加州鲈诺卡菌病病原的分离与鉴定[J].中山大学学报（自然科学版）,2012,51(1):76-81,6.基金项目
广东省省级财政鱼病防治专项资金资助项目(粤财农[2010]347) （粤财农[2010]347）
