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6-BA对黑麦草愈伤组织诱导影响的初步研究

吴莲蓉 徐庆国 李洪 武羿君 黄萍 张婷 赵鑫 李斌 高伟

作物研究2012,Vol.26Issue(1):43-46,4.
作物研究2012,Vol.26Issue(1):43-46,4.DOI:10.3969/j.issn.1001-5280.2012.01.11

6-BA对黑麦草愈伤组织诱导影响的初步研究

Preliminary Studies on Effects of 6- BA on Callus Induction in Ryegrass

吴莲蓉 1徐庆国 1李洪 1武羿君 1黄萍 1张婷 1赵鑫 1李斌 1高伟1

作者信息

  • 1. 湖南农业大学农学院草业科学系,长沙410128
  • 折叠

摘要

Abstract

The mature seeds of three different ryegrass cultivars, perennial ryegrass Yatsyn and Pandora, and annual ryegrass Tetragold, were selected as explants, four types medium of CC, MS, NB, N6 were used, 5 mg/L of 2, 4 - D and different concentration of 6 - BA were added to each medium to study the callus induction in order to establish the regeneration system of ryegrass. The results showed that the callus induction rate of 3 ryegrass cultivars was highest when the concentration of 6 - BA was 0. 05 mg/L. Under the same concentration of 6 - BA, the callus induction rate existed significant differences among different ryegrass varieties. There existed effect of different basic medium on ryegrass callus induction rate, and the ryegrass callus induction rate was highest in CC treatment.

关键词

黑麦草/愈伤组织/6-BA

Key words

Ryegrass/ Callus/ 6 - BA

分类

农业科技

引用本文复制引用

吴莲蓉,徐庆国,李洪,武羿君,黄萍,张婷,赵鑫,李斌,高伟..6-BA对黑麦草愈伤组织诱导影响的初步研究[J].作物研究,2012,26(1):43-46,4.

基金项目

湖南省大学生研究性学习和创新性实验计划项目 ()

中国科学院植物种质创新与特色农业重点实验室开放项目(Y052811t04) (Y052811t04)

湖南省科技厅项目(2010NK3014与10077). (2010NK3014与10077)

作物研究

OACSTPCD

1001-5280

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