作物学报2012，Vol.38Issue(2)：223-230,8.DOI:10.3724/SP.J.1006.2012.00223
绿豆基因组SSR引物在豇豆属作物中的通用性
Transferability of Mungbean Genomic-SSR Markers in Other Vigna Species
摘要
Abstract
Cowpea (Vigna unguiculata L. Walp.), mungbean (V. Radiata L. Wilczek), rice bean [V. Umbellate (Thunb.) Ohwi & Ohashi], and adzuki bean (V; angularis Willd. Ohwi and Ohashi) are major food legumes in China. At present, SSR markers for genetic analysis of these legumes are much limited. Transferability analysis of primers has the vital significance to reduce their development cost and improve their development efficiency. In this study, 1 205 SSR primers were tested for their transferability and polymorphism by PCR amplification with the genomic DNA of four Vigna species, cowpea, adzuki bean, mungbean and rice bean. The results indicated that the transferability rate of mungbean genomic-SSR in cowpea, adzuki bean and rice bean was 50.0%, 73.3%, and 81.6%, and the ratio of polymorphism SSR primers in these crops was 4.1%, 1.7%, and 1.5%, whereas 32.0% in mungbean. A total of 469 mungbean genomic-SSR primers were detected to be highly transferable among different species of Vigna. The transferability of mungbean genomic-SSR was higher in adzuki bean and rice bean than in cowpea. These transferable markers are useful for further genetic and breeding studies in these species.关键词
豇豆属/SSR/通用性Key words
Genus Vigna/SSR/Transferability引用本文复制引用
钟敏,程须珍,王丽侠,王素华,王小宝..绿豆基因组SSR引物在豇豆属作物中的通用性[J].作物学报,2012,38(2):223-230,8.基金项目
本研究由北京市自然科学基金项目(6103025),国家食用豆产业技术体系建设项目(nycytx-18),食用豆行业科技专项(nyhyzx07-017)和农业部作物种质资源保护项目资助(NB10-2130135-25-09)资助. （6103025）
