作物学报2012，Vol.38Issue(2)：285-292,8.DOI:10.3724/SP.J.1006.2012.00285
干旱胁迫下PEPC过表达增强水稻的耐强光能力
High Light Tolerance is Enhanced by Overexpressed PEPC in Rice under Drought Stress
摘要
Abstract
Overexpression of phosphoenolpyruvate carboxylase (PEPC) gene in transgenic rice to improve photosynthesis and stress resistance have brought extensive interest in the world. Our previous report proved that the overexpressed PEPC alleviate drought stress inhibition on photosynthesis. In the present research, photosynthetic light curve, chlorophyll a fluorescence parameters, pigment content and reactive oxygen metabolism were studied under drought stress at flowering stage in two lines of PEPC transgenic rice plants. The results showed that under drought especially severe drought stress, net photosynthetic rate decreased dramatically under high photosynthetic active radiation (PAR) in wild type untransformed rice, while maintained unchanged in PEPC transgenic lines under high PAR even more than 1 200 umol m~2 s-1; and the photochemistry activities (Fv/Fm, ψpsii, and q?) decreased less under drought stress in both the PEPC transgenic lines. These indicated PEPC enhanced the photoinhibition tolerance of rice under drought stress. The higher zeaxanthin content under drought stress made the PEPC transgenic rice leaves disperse more light energy as heat, thus decreased the O2- producing rate in PSII. At the same time, the SOD, POD, and CAT activities were higher in PEPC transgenic rice plants under drought stress. These enzymes could effectively diminished the reactive oxygen species and reduced the membrane lipid peroxidation.关键词
磷酸烯醇式丙酮酸羧化酶/干旱/光抑制/叶绿素荧光参数/活性氧代谢Key words
PEPC transgenic rice/Drought/Photoinhibition/Chlorophyll fluorescence parameter/Reactive oxygen metabolism引用本文复制引用
丁在松,周宝元,孙雪芳,赵明..干旱胁迫下PEPC过表达增强水稻的耐强光能力[J].作物学报,2012,38(2):285-292,8.基金项目
本研究由国家重点基础研究发展计划(973计划)项目(2009CB118605)和国家"十二五"科技支撑计划项目(2011BAD16B14)资助. （973计划）
