作物学报2012，Vol.38Issue(2)：360-368,9.DOI:10.3724/SP.J.1006.2012.00360
非生物胁迫诱导的GmMYB基因克隆与表达分析
Cloning and Expression Analysis of GmMYB Genes Induced by Abiotic Stresses
摘要
Abstract
Response to external environment is the outcome of stress-induced gene expression. In this paper, based on one stress-induced EST sequence, we cloned four R2R3-MYB genes from soybean cultivar Dongnong 42, whose genomic sequences consisted of three exons and two introns. Three of them corresponding to Gm02gl300, Gm03g38040, and Gml0g01340 are respectively consistent with the sequences of Williams 82. A mutation at the 375th single nucleotide in the sequence of Gml9g40650 from Dongnong 42 caused a synonymous amino acid substitution (E125-D125). To test the relationship of four MYB genes with stress resistance, we treated the seedlings of cultivar Dongnong 42 with abiotic stresses including salt, alkali, drought and low temperature in the artificial climate chamber. Quantitative PCR analysis indicated that all of the four genes were transient down-regulated or up-regulated when subjected to the stresses, but different in the expression time, level and tendency. Gm02g01300 was induced by drought stress while Gm03g38040 was strongly induced by multiple stresses, indicating that they play important roles in responding to external stresses. There were also differences in the expression of individual gene between cotyledons and embryos. These results under a variety of abiotic stress conditions suggest that the four R2R3-MYB genes are different not only in the expression patterns, but also in the regulation modes.关键词
非生物胁迫/GmMYB/芽期/表达分析Key words
Abiotic stress/GmMYB/Bud period/Expression analysis引用本文复制引用
孙霞,刘宝辉,刘晋跃,袁晓辉,潘相文,杜维广,任海祥,马永波,JunABE,邱丽娟..非生物胁迫诱导的GmMYB基因克隆与表达分析[J].作物学报,2012,38(2):360-368,9.基金项目
本研究由中国科学院"百人计划"(KZCX2-YW-BR-11),国家自然科学基金项目(30971813和31101170),黑龙江省杰出青年基金(JC200919),黑龙江省青年基金(QC2011C015),黑龙江省归国基金(09SRS11),教育部留学回国人员科研启动基金项目(Y0SQY11001)和所前沿领域项目(2009ZX08009-013B)资助. （KZCX2-YW-BR-11）
