作物学报2012，Vol.38Issue(3)：408-415,8.DOI:10.3724/SP.J.1006.2012.00408
水稻两用核不育系龙S抗稻瘟病主效基因的定位
Mapping of the Resistant Gene to Rice Blast in the Dual Purpose Genic Male Sterile Rice, LongS
摘要
Abstract
LongS is a dual purpose genie male sterile rice with broad-spectrum resistance to rice blast. The objective of the present study was to identify the resistance spectrum to rice blast, to analyze the genetic behavior of resistance gene, and to map the major resistance genes in LongS. LongS had a resistance frequency of 100% inoculated with 41 strains of Magnaporthe oryzae. Population genetic analysis showed that the resistance genes in LongS exhibited dominant inheritance, the genetic model of R gene varied depend on the strains of Magnaporthe oryzae. The main-effect resistant gene to rice blast was fine mapped, by using the bulk segregant analysis (BSA) and recessive class analysis (RCA) methods, with the F2 population derived from the resistant parent of LongS and the susceptible parent of Nipponbare. A single resistant gene to the race of 318-2 located on the interval flanked by the SSR markers of Ml and M2 with a genetic distance of 1.3 cM on chromosome 9 were adjacent to the broad-spectrum blast resistance gene, Pi5. Both of the resistance spectrum and resistant frequency of LongS, however, were significantly different to those of resistant gene of Pi5 and PH. In conclusion, the major-effect resistant gene identified in this study may be a new broad-spectrum blast resistance gene. The DNA markers linked to the new R gene identified in this study should be useful for marker-aided breeding of blast-resistant rice cultivars.关键词
水稻/龙S/稻瘟病/抗性基因/基因定位Key words
Rice/ LongS/ Rice blast/ Resistant gene/ Gene mapping引用本文复制引用
王建龙,贾先勇,王国梁,袁隆平,吴立群,刘建丰,戴良英,刘雄伦,肖应辉,谢红军,刘群恩,李婷..水稻两用核不育系龙S抗稻瘟病主效基因的定位[J].作物学报,2012,38(3):408-415,8.基金项目
本研究由国家高技术研究发展计划(863计划)项目(2010AA101306)和湖南农业大学引进人才基金项目(11YJ14)资助. （863计划）
