作物学报2012，Vol.38Issue(3)：423-428,6.DOI:10.3724/SP.J.1006.2012.00423
水稻卷叶基因RL13的遗传分析和分子定位
Genetic Analysis and Molecular Mapping of a Rolled Leaf Gene RL13 in Rice (Oryza sativa L.)
摘要
Abstract
Rolled leaf mutants are good materials rice in breeding for leaf and plant type. A rolled leaf mutant rl13 was derived from an EMS-treated mutation in an indica line (Jinhui 10). The chlorophyll content of rl13 was significantly higher than that of wild type Jinhui 10. There was a highly significant difference in the curl of the three functional mutant leaves compared with those with wild-type. However, there were no significant differences between the three functional mutants. Through the analysis of paraffin, mesophyll cells per layer in rl13 thinned, wild-type cells contain a large bubble into two similar size vesicular cells of mutants, which resulted in the character of rolled leaf. We made the combination of Xinong 1A/rl13 to establish genetic population for genetic analysis, the results confirmed that the mutant trait was controlled by a single recessive nuclear gene. In the F2, the gene of the rolled leaf trait was mapped between two SSR markers, RM276 and SWU6-1, with distances of 1.1 cM and 0.2 cM, respectively.关键词
水稻(Oryza sativa L.)/卷叶突变体rl13/遗传分析/分子定位Key words
Rice (Oryza sativa L.)/ Rolled leaf mutant/ Genetic analysis/ Molecular mapping引用本文复制引用
田晓庆,桑贤春,赵芳明,李云峰,凌英华,杨正林,何光华..水稻卷叶基因RL13的遗传分析和分子定位[J].作物学报,2012,38(3):423-428,6.基金项目
本研究由重庆市重大攻关项目(CSTC,2010AA1019)和国家转基因生物新品种培育重大专项(2009ZX08009-109B)资助. （CSTC,2010AA1019）
