中药材2012，Vol.35Issue(1)：102-105,4.
开口箭皂苷抗内毒素作用的实验研究
Study on the Anti-endotoxin Effect of Saponin from Tupistra chinensis
摘要
Abstract
Objective;To investigate the effect of saponin from Tupistra chinensis Baker(STCB)on lethal toxicity of endotoxin in mice and explore the underlying mechanism. Methods: Mouse models of endotoxin-induced death and endotoximia were established byintraperitoneal administration of KM mice with lipopolysaccharides ( LPS) from Pseudomonas aeruginosa in doses of 60 mg/kg and 10 mg/kg respectively. Mouse survival rate and survival time were recorded and the serum levels of interleukin-lβ(IL-l/6)and tumor necrosis factor-a( TNF-a) in endotoximia mice were measured by enzyme-linked immunosorbent assay (ELISA). Mouse peritoneal exudate cells induced by LPS were used as an in vitro inflammatory model,which was then intervened with STCB and the levels of IL -1/3 and TNF-a in the culture supernatants were measured by ELISA. Results: The survival rates of mice prophylactically treated with STCB (200 and 400 mg/kg,in 5 consecutive days) were slightly higher compared with that in model group, but no statistical difference was observed ( P＞0. 05 ). The survival time was much longer in the treated group ( P＜0. 05 ). The serum levels of IL-1β and TNF-a in STCB-treated mice( 200 and 400 mg/kg, in 5 consecutive days) were significantly lower compared with those in model group (P＜0.05). STCB (20 and 40 μg/mL) remarkably inhibited LPS-induced IL-lβ and TNF-a production by peritoneal exudate cells in vitro(P＜0. 05). Conclusion:Saponin from Tupistra chinensis showed beneficial effect on the prevention of mice from lipopolysacchar-ides-induced death, in which down regulation of IL-1β and TNF-α expression might be involved.关键词
开口箭/皂苷/内毒素/白介素1β/肿瘤坏死因子αKey words
Tupistra chinensis Baker/Saponin/Endotoxin/IL-lβ/TNF-α分类
医药卫生引用本文复制引用
任婧,雷林生,余传林,何概易南,陈娜娜..开口箭皂苷抗内毒素作用的实验研究[J].中药材,2012,35(1):102-105,4.基金项目
广东省自然科学基金(07005189) （07005189）
