癌变·畸变·突变2012，Vol.24Issue(2)：81-85,5.DOI:10.3969/j.issn.1004-616x.2012.02.001
N-乙酰半胱氨酸对支气管上皮细胞辐射致癌模型DNA氧化损伤和存活率的影响
Effects of N-acetyl cysteine on the oxidative DNA damage and cell survival rate of malignant BERP35T-1 cells exposed to α-particles
摘要
Abstract
OBJECTIVE:To study the effects of N-acetyl cysteine (NAC) on the oxidative DNA damage and cell survival rate of malignant BERP35T-1 cells exposed to α -particles. METHODS: Western blot was used for the detection of protein expression of γ H2AX in the human bronchial epithelial cell lines BEP2D, RH22 and BERP35T-1, and BERP35T-1 treated with 0-2 mmol/L NAC for 48 h. Immunocytochemistry was used to compare the different expression levels of 8-OH-dG in BERP35T-1 treated with 1 mmol/L NAC for 0-48 h. Using DCHF-DA, the generation of active oxygen radicals (ROS) in BERP35T-1 treated with 1 mmol/L NAC for 48 h was monitored by flow cytometry. MTT assay was used to examine the cell survival rate of BERP35T-1 cells treated with 1 mmol/L NAC for 48 h and/or 2 Gy γ -rays. RESULTS: Compared to BEP2D cells, increased level of γ H2AX was detected in RH22 and BERP35T-1 cells (P<0.01). The protein expression of γ H2AX in BERP35T-1 was higher than in RH22 cells (P<0.01). After treatment with 1-2 mmol/L NAC for 48 h, the expression level of γH2AX in BERP35T-1 was significantly decreased (P<0.01). Decreased expression of 8-OH-dG was seen in BERP35T-1 treated with 1 mmol/L NAC for 24-48 h (P<0.05 or P<0.01). After treatment by 1 mmol/L NAC for 48 h, the basal level of ROS in BERP35T-1 decreased (P<0.05). In addition, the cell survival rate of BERP35T-1 treated with NAC was reduced (P<0.01). Meanwhile, compared to BERP35T-1 treatment with γ -rays alone, increased cell survival rate was detected in BERP35T-1 when treated with γ -rays combined with NAC(P<0.05). CONCLUSION: NAC, as an antioxidant, could up-regulate the antioxidant ability of BERP35T-1, resulting in decreased ROS level and oxidative DNA damage and increased genomic stability to partially reverse the malignant phenotype of BERP35T-1, such as inhibiting the malignant proliferation, and reducing its radiosensitivity.关键词
N-乙酰半胱氨酸/α粒子/肺癌/氧化损伤/细胞存活率Key words
N-acetyl cysteine/α-particle/lung cancer/oxidative damage/cell survival rate分类
生物科学引用本文复制引用
苟巧,王春燕,佟鹏,吕慧敏,齐雪松,郝述霞,魏志权..N-乙酰半胱氨酸对支气管上皮细胞辐射致癌模型DNA氧化损伤和存活率的影响[J].癌变·畸变·突变,2012,24(2):81-85,5.基金项目
国家自然科学基金项目(81000862) （81000862）
