癌变·畸变·突变2012，Vol.24Issue(2)：96-99,107,5.DOI:10.3969/j.issn.1004-616x.2012.02.004
应用质谱技术检测结肠癌细胞DPYD和MTHFR基因单核苷酸多态性
Detection of single nucleotide polymorphisms of DPYD and MTHFR genes in colon cancer cell lines
摘要
Abstract
OBJECTIVE: This study was purposed to identify single nucleotide polymorphisms (SNPs) of two pharmacokinetics-related genes in two colon cancer cell lines. METHODS: HT-29 and Lovo cell lines were cultured. The genomic DNA of the two cell lines were isolated by QIAamp DNA Blood Mini kit. Then we designed the primers and amplified the related DNA fragments by PCR. SNP genotyping of DPYD gene rs1801159(A/G), rs1801160 (G/A), rsl7376848 (A/G) and MTHFR gene rs2274976 (G/A), rsl801131 (A/C) and rs1801133 (C/T) was performed by means of matrix assisted laser desorption ionisation-time of flight mass spectrometry method (MALDI-TOFMS). RESULTS: The genotypes of DPYD gene locus rs1801159, rsl801160, rsl7376848 were A/A, G/G and A/A, respectively, in the two cell lines. The genotype of MTHFR gene locus re1801131, rs1801133 and rs2274976 were A/C, C/T and G/G, respectively, in HT-29 cell line. While those were A/A, T/T and G/A, respectively, in Lovo cell line. CONCLUSION: The above-mentioned loci of DPYD were the same in HT-29 and Lovo cell lines. However the detected loci of MTHFR gene in the two cell lines were expressed differently.关键词
结肠癌/单核苷酸多态性/DPYD/MTHFRKey words
colon cancer/ single nucleotide polymorphisms/ DPYD/ MTHFR分类
生物科学引用本文复制引用
陈宝安,彭苗新,程璐,陆祖宏,张文静,张孝平,柏志斌,冯继峰,仲悦娇,丁家华,高冲,鲍文..应用质谱技术检测结肠癌细胞DPYD和MTHFR基因单核苷酸多态性[J].癌变·畸变·突变,2012,24(2):96-99,107,5.基金项目
国家自然科学基金项目(30872970,30740062) （30872970,30740062）
江苏省科技厅重大项目(EM2006111) （EM2006111）
