癌变·畸变·突变2012，Vol.24Issue(2)：116-120,5.DOI:10.3969/j.issn.1004-616x.2012.02.008
内质网应激在芹菜素诱导胃癌细胞凋亡中的作用
Roles of endoplasmic reticulum stress in apigenin-induced apoptosis in human gastric cancer SGC-7901 cells
王晓琳 1黄晓莉 1赵艳 1张志宏 1张旭光 1吕和 1吴坤1
作者信息
- 1. 哈尔滨医科大学公共卫生学院营养与食品卫生学教研室,黑龙江哈尔滨150081
- 折叠
摘要
Abstract
OBJECTIVE: To investigate the roles of endoplasmic reticulum stress (ERS) in apigenin (API) -induced apoptosis in human gastric cancer SGC-7901 cells. METHODS: SGC-7901 cells were treated with API at 20, 40, 60 and 80 μ mol/L and at 3 μ g/ml tunicamycin (TM) for 48 h or at 60 μ mol/L for up to 48 h. GRP78, GRP94 and C/EBP-homoIogous protein(CHOP) were explored using Western blot. When the cells were treated with endoplasmic reticulum stress inhibitor 4-phenyl butyric acid (4-PBA ), apoptosis was assessed by DAPI staining and flow cytometry. RESULTS: The protein levels of GRP78 and GRP94 increased in API-treated cells in time and dose -dependent manner, but apigenin did not induce the expression of CHOP protein. Pretreatment with 4-PBA drastically increased API-induced apoptosis in SGC-7901 cells (P<0.01). CONCLUSION: ERS may exert protective effect on API-induced apoptosis in human gastric cancer SGC-7901 cells.关键词
内质网应激/芹菜素/细胞凋亡Key words
endoplasmic reticulum stress/ apigenin/ apoptosis分类
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王晓琳,黄晓莉,赵艳,张志宏,张旭光,吕和,吴坤..内质网应激在芹菜素诱导胃癌细胞凋亡中的作用[J].癌变·畸变·突变,2012,24(2):116-120,5.
