癌变·畸变·突变2012，Vol.24Issue(2)：121-124,128,5.DOI:10.3969/j.issn.1004-616x.2012.02.009
免疫杀手细胞的体外扩增方法及其对肝癌细胞BEL-7402的杀伤作用
Evaluation of a modified culture method for immune-killer cells and therapeutic efficiency on hepatocarcinoma cell line BEL-7402
摘要
Abstract
OBJECTIVE:To evaluate the modified culture method and the therapeutic efficiency of immune-killer cells (IKCs) on a hepatocarcinoma cell line BEL-7402. METHODS: IKCs were induced and amplified from PBMC cells in a modified NK culture method. The immunophenotype changes were analyzed by flow cytometry. The proliferation of the cells were evaluated by Trypan-blue staining. The killing activities on BEL-7402 cell line were detected by Alamar Blue staining and their effect on BEL-7402 tumor in the nude mice model were examined by monitoring tumor growth. RESULTS: The IKCs cultured for 14 days- multiplied approximately 300 times. Cell subsets of CD3+CD56+, CD3-CD56+ and CD8+ were the major components. Both ratios of CD3+CD56+ and CD3-CD56+ were higher than 85%, while the ratio of CD8+ was higher than 77%. At the effector-target ratio of 25: 1 and 100: 1, the killing activity against BEL-7402 cells of IKCs was 66.5% (P< 0.01) and 97.8% (P< 0.01), respectively. Treatment with IKCs on BEL-7402 tumor in nude mice showed a noticeable higher therapeutic effect than that of control, with an inhibition ratio on tumor growth of 69% (P< 0.01). CONCLUSION: The modified culture method for IKCs was practicable and effective. The research might provide experimental basis for clinical immunotherapy of IKCs on hepatocarcinoma.关键词
免疫杀手细胞/肝癌/杀伤Key words
immune-killer cells/ hepatocarcinoma/ killing activity分类
医药卫生引用本文复制引用
王生育,罗芳洪,倪二茹,吕海燕,支黎明,颜江华,张顺浪..免疫杀手细胞的体外扩增方法及其对肝癌细胞BEL-7402的杀伤作用[J].癌变·畸变·突变,2012,24(2):121-124,128,5.基金项目
厦门长春堂科技有限公司体细胞研究与应用项目(XDHT2007001C) （XDHT2007001C）
福建省科技专项(2009R10020-1) （2009R10020-1）
