安徽农业大学学报2012，Vol.39Issue(2)：207-210,4.
高效毛细管电泳法同时分离氟喹诺酮类与四环素类药物的研究
Separation and determination of fluoroquinolones and tetracyclines by high performance capillary electrophoresis
摘要
Abstract
A method for the simultaneous separation of fluoroquinolones (FQs) and tetracyclines (TCs) by high performance capillary electrophoresis(HPCE) was developed. The effects of several factors such as the con centration and pH of running buffer, the organic added to the electrolyte, the voltage and the temperature on sepa ration were investigated. The optimal conditions were as follows. The UV detection wavelength was 262 nm. The running butter consisted of 50 mmol·L-1 NaB4O7-200 mmol·L-1 H3BO3 with pH 8.41, and the separation voltage was at 18 kV. The results showed that the six remedies can be well separated within 12 min. Each had a good lin ear relationship (R2=0.998 2~l).The RSD was 0.25%~0.68% and 7.3%~9.8%.关键词
高效毛细管电泳/氟喹诺酮/四环素/同时分离Key words
HPCE/ FQs/ TCs/ simultaneous separation分类
农业科技引用本文复制引用
沈虎琴,檀华蓉,祁克宗,田春秋..高效毛细管电泳法同时分离氟喹诺酮类与四环素类药物的研究[J].安徽农业大学学报,2012,39(2):207-210,4.基金项目
“十一五”国家科技支撑项目(2006BAK02A08-4)资助. （2006BAK02A08-4）
