安徽农业大学学报2012，Vol.39Issue(2)：273-279,7.
野大豆等宿主根瘤菌表型及16S rDNA PCR-RFLP研究
Phenotypic analysis and 16S rDNA PCR-RFLP of rhizobial strains isolated from Glycine max etc
摘要
Abstract
Sixty strains obtained from root nodules of Glycine soja sieb, Glycine max, Phaselous vulgaris and Kummerowiae stipulacea of Beijing were studied with numerical taxonomy and 16S rDNA PCR-RFLP. Results of numerical taxonomy indicated that all strains included 10 rhizobium reference strains were divided into 3 groups at 70.5% similarity. Group I is an unknown group with no reference strains. Group II belongs to fast and mid dle-slow-growing type, and group III belongs to slow-growing kind. The dendrogram derived from 16S rDNA PCR-RFLP showed that all strains divided into nine phylogenetic branches at the similarity of 69%. They are branches I, V, VI, VII, VIII and XI with no reference strains, II (Sinorhizobium -Mesorhizobium- Rhizobium), Ⅲ(Agrobacterium) and IV (Bradyrhizobium).关键词
根瘤菌/数值分类/16S rDNA/PCR-RFLPKey words
Rhizobium/ numerical taxonomy/ 16S rDNA/ PCR-RFLP分类
生物科学引用本文复制引用
王静,刘磊,梁芳,欧明丽,刘少佳,胡玉莹,杨帆,韩素贞..野大豆等宿主根瘤菌表型及16S rDNA PCR-RFLP研究[J].安徽农业大学学报,2012,39(2):273-279,7.基金项目
北京市教委基金(KM200510082008)资助. （KM200510082008）
