安徽农业大学学报2012，Vol.39Issue(3)：397-400,4.
茶树花粉特异蛋白基因CsPSP的反义载体构建
Antisense vector construction of CsPSP pollen specific protein gene from tea plant (Camellia sinensis L.)
摘要
Abstract
In order to study the expression of the pollen-specific gene in tea plant, a pair of specific primers with double enzyme sites was designed to clone a Cdna fragment of CsPSP gene from Longjing43 by the method of RT-PCR, and then the CsPSP fragment was ligated with Pbi121 vector and sequenced. The result indicates that the sequence has 99.02% similarity to CsPSP gene in GenBank.关键词
茶树/CsPSP/反义载体Key words
Camellia sinensis./ CsPSP/ antisense vector分类
农业科技引用本文复制引用
龚莹,余梅,江昌俊,吴凡,吉虎,徐凯,臧洁..茶树花粉特异蛋白基因CsPSP的反义载体构建[J].安徽农业大学学报,2012,39(3):397-400,4.基金项目
安徽高校省级自然科学研究重点项目计划(KJ2010A119)资助. （KJ2010A119）
