安徽农业大学学报2012，Vol.39Issue(3)：478-482,5.
二次通用旋转组合设计优化辣椒SRAP-PCR反应体系
Optimization of SRAP-PCR system in pepper by quadratic general rotary unitized design
谈敏 1刘童光 1张其安 2方凌2
作者信息
- 1. 安徽农业大学园艺学院,合肥230036
- 2. 安徽省农业科学院园艺研究所,合肥230031
- 折叠
摘要
Abstract
The quadratic general rotary unitized design was used to optimize SRAP-PCR system in pepper (Capsicum spp.) with five key factors (Taq DNA polymerase, Mg2+, dNTPs, primer and template DNA, respec-tively).The results showed that the optimized SRAP-PCR system for pepper was: 0.5 U Taq DNA polymerase, 2.3 mmol-L"1 Mg2^ 0.2 mmol-L"1 dNTPs, 0.8 umol-L'1 primer, 45 ng template DNA in a total of 10 uL reaction solution. The optimized SRAP-PCR system was tested on 18 pepper germplasm and was reliable and steady. Accordingly, the method of the test and the optimized SRAP-PCR system were suitable for SRAP (sequence-related amplified polymorphism) analysis of pepper.关键词
辣椒/SRAP/二次通用旋转组合设计/体系优化Key words
pepper/ SRAP/ quadratic general rotary unitized design/ system optimization分类
农业科技引用本文复制引用
谈敏,刘童光,张其安,方凌..二次通用旋转组合设计优化辣椒SRAP-PCR反应体系[J].安徽农业大学学报,2012,39(3):478-482,5.
