安徽农业科学2012，Vol.40Issue(10)：5743-5745,5830,4.
重组融合蛋白最佳诱导表达条件的研究
Research on Best Induced Conditions for Expression of Recombinant Fusion Protein
摘要
Abstract
[Objective] To study the best induced conditions for expression of recombinant fusion protein. [ Method] The best initial OD600, induction amount, induction duration, induction temperature, ampicillin concentration, liquid volume and other culture conditions were explored by using parallel fermentation experiments with different technical indicators to improve the expression level of the recombinant fusion protein of IMPACT-CN pTYBl 1 vector. [ Result] Expression of SDS-PACE and Weatem-Blotting analysis showed that the recombinanl fusion protein has a high immunogenictty. [ Conclusion ] This study laid the foundation for further protein purification in diagnosis, production of diagnostic kits with protein and clinical application.关键词
克隆/表达/融合蛋白Key words
Cloning/ Expression/ Fusion protein分类
农业科技引用本文复制引用
毕智丽..重组融合蛋白最佳诱导表达条件的研究[J].安徽农业科学,2012,40(10):5743-5745,5830,4.基金项目
滨州职业学院科研项目(07XYA04). （07XYA04）
