安徽农业科学2012，Vol.40Issue(10)：5751-5754,4.
甜瓜离体再生体系优化研究
Research on in vitro Regeneration System of Melon
摘要
Abstract
[Objective] This study aimed to optimize the in vitro regeneration system of melon. [Method] Melon variety Nanxiang91023 was selected as experimental materials, with the cotyledons and hypocotyls as explants, different types and concentrations of growth regulators were supplemented at different stages of tissue culture, to explore the simple and effective medium formula for regeneration of melon. [ Result ] MS + 1.5 mg/L of 6-BA + 0.2 mg/L of IAA was the optimal medium for induction and proliferation of callus; MS + 1. 0 mg/L of 6-BA + 0. 2 mg/L of 2,4-D was the optimal medium for differentiation of adventitious buds; MS + 1.0 mg/L of ZT + 0.2 mg/L of IAA was the optimal medium for rooting of seedlings. On the basis of above conditions, melon seedlings had high rooting rate and strong roots. [ Conclusion ] This study provided a guarantee for the further genetic transformation of improved melon varieties.关键词
甜瓜/再生体系/下胚轴/子叶Key words
Melon/ Regeneration system/ Hypocotyls/ Cotyledons分类
农业科技引用本文复制引用
赵燕,刘清波,任春梅,马珊珊,黄红梅..甜瓜离体再生体系优化研究[J].安徽农业科学,2012,40(10):5751-5754,4.基金项目
湖南省科技厅资助项目(2010FJ3103). （2010FJ3103）
