盐生植物盐穗木HcPS_H基因大肠杆菌表达载体的构建OA
Constructing of a E.coli Expression Vector of HcPS_H Gene from Halostachys caspica
[目的]构建盐穗木HcPS_H基因的大肠杆菌表达载体.[方法]以质粒pGEM-T- HcPS_H为模板,HcPS_H-pET28a-F、HcPS_H-pET28a-R为引物进行PCR扩增,获得两端含酶切位点的HcPS_H基因目的片段.将该目的片段与大肠杆菌表达载体pET-28a通过T4DNA连接酶连接,并转化大肠杆菌(Escherichia coli)DH5α,得到重组子克隆,抽质粒进行测序鉴定.[结果]扩增出HcPS_H基因片段长度为441 b…查看全部>>
[ Objective ] To construct a E. Coli expression vector of HcPS_H gene from Hahstachys caspica. [ Method ] The HcPS_H gene fulllength fragment was obtained from pGEM- T- HcPS_H by PCR using a pair of including enzyme site primer, and then the purified fragment was cloned into pET-28a vector. [ Result]HcPS_H gene was amplified from Hahstachys caspica. The gene fragment was 441 bp,and was cloned into pET-28a vector. After analysis of restriction endonuclease di…查看全部>>
郝晓燕;张毓露;足木热木;刘小利
新疆农业科学院核技术生物技术研究所,新疆乌鲁木齐830091新疆农业大学农学院,新疆乌鲁木齐830052新疆农业科学院核技术生物技术研究所,新疆乌鲁木齐830091新疆农业大学农学院,新疆乌鲁木齐830052
农业科技
盐生植物盐穗木HcPS_H基因原核表达载体
HalophytesHalostachys caspica HcPS-H gene E. Coli expression vector
《安徽农业科学》 2012 (10)
5758-5759,2
新疆维吾尔自治区农作物生物技术重点实验室开放课题(XJDX0201-2011-05).
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