安徽农业科学2012，Vol.40Issue(10)：5758-5759,2.
盐生植物盐穗木HcPS_H基因大肠杆菌表达载体的构建
Constructing of a E.coli Expression Vector of HcPS_H Gene from Halostachys caspica
摘要
Abstract
[ Objective ] To construct a E. Coli expression vector of HcPS_H gene from Hahstachys caspica. [ Method ] The HcPS_H gene fulllength fragment was obtained from pGEM- T- HcPS_H by PCR using a pair of including enzyme site primer, and then the purified fragment was cloned into pET-28a vector. [ Result]HcPS_H gene was amplified from Hahstachys caspica. The gene fragment was 441 bp,and was cloned into pET-28a vector. After analysis of restriction endonuclease digestion and PCR, the constructed expression vector was proved to be correct. [ Conclusion] The expression vector of HcPS_H gene of Hahstachys caspica was constructed successfully.关键词
盐生植物/盐穗木/HcPS_H基因/原核表达载体Key words
Halophytes/Halostachys caspica/ HcPS-H gene/ E. Coli expression vector分类
农业科技引用本文复制引用
郝晓燕,张毓露,足木热木,刘小利..盐生植物盐穗木HcPS_H基因大肠杆菌表达载体的构建[J].安徽农业科学,2012,40(10):5758-5759,2.基金项目
新疆维吾尔自治区农作物生物技术重点实验室开放课题(XJDX0201-2011-05). （XJDX0201-2011-05）
