安徽农业科学2012，Vol.40Issue(10)：5773-5774,2.
山苍子外植体初始诱导的研究
Research on Initial Explants Induction of Litsea cubeba
吕曼芳 1周传明 1周如琼 1熊英2
作者信息
- 1. 广西大学林学院,广西南宁530004
- 2. 广西大学农学院,广西南宁530004
- 折叠
摘要
Abstract
[ Objective ] The aim of this study was to explore an explants induction method for L. Cubeba. [ Method ] Different types of L. Cube-ba stem segments were collected at different time as explants materials for induction and culture. [ Result ] The results showed that sterilization with 0. 1% HgCl2 for 10 min achieved better effects, with an explants contamination rate of 40.0% and a mortality rate of 15.0% ; stem segments of the 2-5 buds below terminal bud were better explants materials for initial induction; the best sampling time was January, with an induction rate as high as 81.1 % , while the contamination rate was only 11.7%; modified MS + 1.0 mg/L of 6-BA +0.2 mg/L of NAA was the optimal medium for explants induction of L cubeba. [ Conclusion ] Semi-lignified stem segments of L. Cubeba collected in January, sterilized with 0. 1 % HgCl2 for 10 min and cultured in modified MS + 1.0 mg/L of 6-BA +0.2 mg/L of NAA had achieved better induction effects on L cubeba seedlings.关键词
山苍子/外植体/消毒/诱导培养Key words
Litsea cubeba/ Explants/ Sterilization/ Induction分类
农业科技引用本文复制引用
吕曼芳,周传明,周如琼,熊英..山苍子外植体初始诱导的研究[J].安徽农业科学,2012,40(10):5773-5774,2.
