安徽农业科学2012，Vol.40Issue(10)：5776-5778,3.
红芝LYL263所产漆酶在溶胶-凝胶体系中的固定化研究
Immobilization of Laccase from Ganoderma lucidum LYL 263 in Sol-gel System
摘要
Abstract
[ Objective ] The aim was to study the immobilization of laccase from Canoderma lucidum in three different sol-gel systems to find out the optimum materials for laccase immobilization. [Method] Laccase from G. Lucidum LYL 263 was immobilized in sodium alginate-gelatin, sodium alginate-chitosan and sodium alginate-gelatin-chitosan sol-gel systems, respectively; and the laccase activity was preliminarily analyzed. [Result] The activity of laccase immobilized in sodium alginate-gelatin-chitosan was 2.14 folds and 2.75 folds respectively, compared to sodium alginate-gelatin and sodium alginate-chitosan; and this optimum system contained sodium algiiiate 2.0% , gelatin 1.0% , chitosan 0.3% and calcium chloride 36%. Benzoic acid was firstly used to embed immobilized laccase in this study, and single-factor experiments revealed that the activity of laccase could be effectively improved by suitable content of benzoic acid. The optimal conditions for laccase immobilization in the system were benzoic acid 2 mmol/L, glutaraldehyde 0.32% , cross-linking reaction time 50 min and enzyme concentration 10.0% ; and then the activity of the immobilized enzyme reached 635.7 U/g with these conditions. The thermal stability of the immobilized laccase was higher than free laccase, and the activity of immobilized enzyme treated by freeze-drying was 1.59 folds compared to the non-freeze-dried, but the freeze-dried immobilized laccase had poor operational stability. [Conclusion] The compositions of the material had significant influence on laccase immobilization and this study will provide reference to select materials for immobilization of laccase from C. Lucidum LYL 263.关键词
红芝LYL263漆酶/固定化/苯甲酸/溶胶-凝胶体系/冻干Key words
Laccase/ Immobilization/ Benzoic acid/ Sol-gel/ Freeze-drying分类
农业科技引用本文复制引用
陆兵,谌斌,易小畅,李娜,殳静..红芝LYL263所产漆酶在溶胶-凝胶体系中的固定化研究[J].安徽农业科学,2012,40(10):5776-5778,3.基金项目
广西自然科学基金面上项目(2011GXNSFA018080) （2011GXNSFA018080）
广西教育厅面上科研项目(桂教科研[2009]25号) （桂教科研[2009]25号）
广西环境工程与保护评价重点实验室平台建设二期项目资助. （）
