安徽农业科学2012，Vol.40Issue(10)：5782-5785,4.
无核荔枝半胱氨酸蛋白酶抑制剂基因克隆及序列分析
Cloning and Sequence Analysis of a Cysteine Proteinase Inhibitor Gene of Seedless Litchi
刘兴地 1刘娜 2李明芳 1郑学勤1
作者信息
- 1. 中国热带农业科学院热带生物技术研究所,海南海口571101
- 2. 海南大学,海南海口571101
- 折叠
摘要
Abstract
[ Objective] This study aimed to clone the cysteine proteinase inhibitor gene of seedless litchi and analyze the sequence. [ Method] According to the EST sequence of cysteine proteinase inhibitor in constructed SSH subtractive library of seedless litchi abortion, nucleotide sequence of the cysteine proteinase inhibitor gene was obtained by using RACE technology and analyzed by using bioinformatics software. [ Result] A cysteine protease inhibitor gene was obtained with the sequence of 635 bp containing a 321 bp open reading frame. It was predicted that the encoded protein contained 106 amino acids with conserved domain of cysteine proteinase inhibitor and had relatively high homology with the cysteine proteinase inhibitor gene of several species. [ Conclusion] This study had laid the foundation for further exploring the physiological functions of this cysteine proteinase inhibitor gene in plants.关键词
无核荔枝/半胱氨酸蛋白酶抑制剂/克隆/序列分析Key words
Seedless litchi/Cysteine proteinase inhibitor/Cloning/ Sequence analysis分类
农业科技引用本文复制引用
刘兴地,刘娜,李明芳,郑学勤..无核荔枝半胱氨酸蛋白酶抑制剂基因克隆及序列分析[J].安徽农业科学,2012,40(10):5782-5785,4.
