安徽农业科学2012，Vol.40Issue(10)：5806-5807,2.
一种广泛适用于植物基因组DNA提取的方法
A Widely Applicable Method for Plant Genomic DNA Extraction
摘要
Abstract
[ Objective ] This study aimed to explore a method for plant genomic DNA extraction, to provide guidance for rapid extraction of ge-nomic DNA from plant tissues. [ Method] Based on the published genomic DNA extraction methods, operation steps, reagent amount and processing time of SDS extraction method were optimized. [ Result] A widely applicable method was established initially for genomic DNA extraction from various varieties of plants and various kinds of plant tissues. Quality of the extracted genomic DNA was relatively good, which met the requirements for further operation. [Conclusion] This study provided guidance for rapid plant genomic DNA extraction.关键词
植物基因组/DNA提取/优化SDS提取法Key words
Plant genome/ DNA extraction/ Optimized SDS extraction method分类
农业科技引用本文复制引用
李斌,于丽霞,习珺珺,张亚萍,高则睿,鄢波..一种广泛适用于植物基因组DNA提取的方法[J].安徽农业科学,2012,40(10):5806-5807,2.基金项目
国家自然科学基金(31060042) （31060042）
云南省应用基础研究项目资助(2009ZC079M). （2009ZC079M）
