安徽农业科学2012，Vol.40Issue(10)：5870-5872,3.
不同提取方法对桦褐孔菌多糖抗氧化活性的影响
Effects of Different Extraction Methods on Antioxidant Activity of Inonotus obliquus Polysaccharides
摘要
Abstract
[ Objective ] This study aimed to explore the effects of different extraction methods on antioxidant activities of /. Obliquus polysaccharides. [ Method ] Crude polysaccharides were extracted by using boiling ethanol precipitation method and ethanol-alkali extraction method; water-soluble and alkali-soluble polysaccharides were purified by using trichloroacetic acid method; antioxidant activities of /. Obliquus polysaccharides were compared by hydroxyl radical scavenging experiment and superoxide anion radical scavenging experiment. [ Result] Yield of crude water-soluble polysaccharides, crude alkali-soluble polysaccharides, pure water-soluble polysaccharides and pure alkali-soluble polysaccharides were 14.0% , 27.7% , 7.0% and 6.2% , respectively. Hydroxyl radical scavenging effect of crude water-soluble polysaccharides and pure alkali-soluble polysaccharides had achieved the maximum with polysaccharide concentration of 0.9 mg/ml and 0.06 mg/ml, and the scavenging rates were 54. 3% and 10.9% , respectively; superoxide anion radical scavenging effect of crude water-soluble polysaccharides and pure water-soluble polysaccharides had achieved the maximum with polysaccharide concentration of 0. 1 mg/ml and 0. 07 mg/ml, and the scavenging rates were 24. 2% and 27. 2% , respectively. [ Conclusion] Antioxidant activity of water-soluble polysaccharides was higher than that of alkali-soluble polysaccharides. /. Obliquus could be used as a species of new efficient natural antioxidant medicinal fungi in application.关键词
桦褐孔菌多糖/得率/自由基/清除/抗氧化Key words
Inonolus obliquus polysaccharide/ Yield/ Free radical/ Scavenging/ Antioxidant分类
农业科技引用本文复制引用
张丽霞,张伟娜,李凌智,张虹..不同提取方法对桦褐孔菌多糖抗氧化活性的影响[J].安徽农业科学,2012,40(10):5870-5872,3.基金项目
黑龙江省教育厅科学技术研究指导项目(11533005). （11533005）
