RP-HPLC法测定颈复康颗粒中葛根素的含量OA
The Determination of the Content of Puerain in Neck-Recover-Granule Using RP-HPLC
[目的]采用反相高效液相色谱法测定颈复康颗粒中葛根素的含量.[方法]色谱柱为Kromasil 100-5C18(250 mm×4.6mm,5μm)；流动相为无水甲醇-水-磷酸(20∶80∶0.2,V/V/V),流速为1.0 ml/min;检测波长为250 nm.[结果]阴性对照无干扰；计算回归方程为Y=4 233 801X -34 587,R为0.999 9(n =6).结果表明,在0.063～1.254μg/ml浓度范围内,葛根素浓度与峰面积呈…查看全部>>
[ Objective ] The aim was to determine the content of puerarin in neck-recover-granule using RP-HPLC. [ Methods ] The chromato-graphic column was Kromasil 100-5C18(250 mm x4.6 mm,5 μm) jmobile phase was absolute methanol-water-phosphoric acid (20:80:0.2, V/ V/V), flow velocity was 1.0 ml/min; the detection wavelength was 250 nm. [ Result ] The negative control showed no interference, the calculated regression equation was Y = 4 233 801X - 34 587, R = 0. 999 …查看全部>>
李红芳;韩继永
江苏联合职业技术学院连云港中医药分院,江苏连云港222006江苏奥赛康药业有限公司,江苏南京211112
农业科技
HPLC颈复康颗粒葛根素含量
HPLC Neck-recover-granule Puerain Content
《安徽农业科学》 2012 (10)
5883,5894,2
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