安徽农业科学2012，Vol.40Issue(10)：5981-5983,3.
多胺处理对小鼠子宫内膜上皮细胞的影响
Effect of Polyamine Treatment on Mouse Endometrial Epithelial Cells
摘要
Abstract
[ Objective ] The aim of this study was to investigate the effect of polyamine treatment on mouse endometrial epithelial cells. [ Method ] After the mouse endometrial epithelial cells were treated with low levels of putrescine, spermidine and spermine respectively, the transcription of polyamine-related genes was detected by real-time PCR, and cell proliferation was disclosed by cell counting, MTT and flow cytometry analysis. [Result] Putrescine obviously decreased the mRNA levels of S-adenosylmethionine decarboxylase (SAMDC), spermine oxidase (SMO), spermine synthase(SMS), ornithine decarboxylase (ODC) and polyamine oxidase (PAO) genes. Spermidine obviously decreased the mRNA levels of SAMDC, spermidine synthase (SRM) and ODC genes and obviously increased the mRNA levels of spermidine/ spermine Nl-acetyltransferase ( SSAT) genes. Spermine obviously decreased the mRNA levels of SAMDC, SRM and ODC genes and obviously increased the mRNA levels of SMO, PAO and SSAT genes. The epithelial cells viability was significantly increased after treatment with putrescine and spermidine, but it was decreased by spermine treatment. [ Conclusion ] Regulation of polyamine-related genes by polyamine could lead to the changes of polyamine-related gene mRNAs, which might regulate the epithelial cell proliferation.关键词
多胺/小鼠/子宫内膜上皮细胞/多胺代谢相关基因/细胞增殖Key words
Polyamine/ Mouse/ Endometrial epithelial cell/ Polyamine-related gene/ Cell proliferation分类
农业科技引用本文复制引用
罗晓彤,朴庆林,于永生..多胺处理对小鼠子宫内膜上皮细胞的影响[J].安徽农业科学,2012,40(10):5981-5983,3.基金项目
国家重大科学研究计划项目(2006CB504005) （2006CB504005）
吉林省博士后科研启动基金(00220) （00220）
吉林省农业科学院引进人才基金(00109)资助. （00109）
