SSR标记在油菜隐性核不育杂交种的纯度鉴定研究OA
Hybrid Purity Identification of Genic Male Sterile by SSR
[目的]利用SSR标记对油菜隐性核不育杂交种的纯度进行鉴定.[方法]以贵州省油菜科学研究所选育的甘蓝型油菜“油研系列”为材料,利用简化的SSR标记技术对制种样品进行纯度分析.[结果]筛选出了2对条带清晰、易于统计的SSR引物；大田生产F1杂种群体的纯度鉴定结果表明,SSR鉴定的结果清晰可辩,可信度高,能较准确地反映种子的真实纯度.[结论]该试验为SSR标记在油菜种子纯度鉴定中的进一步应用奠定了基础.
[ Objective ]The aim was to identify the hybrid purity of rape genie male sterile by SSR makers. [Method] To analyze the purity of aeedsamples by simplified SSR markers, using the Brassica napus " Yujan aeries" as materials which had been nurtured by Guizhou Institute of OilCrops. [Result]2 primers were selected according to PIC (polymorphism information content) value and the result of F, purity identification,which produced in field, showed that the SSR me…查看全部>>
张宁洁;陈丽;朱文秀;曾章丽;王仙萍
贵州省油菜研究所,贵州贵阳550001贵州省油菜研究所,贵州贵阳550001贵州省油菜研究所,贵州贵阳550001贵州省油菜研究所,贵州贵阳550001贵州省油菜研究所,贵州贵阳550001
农业科技
甘蓝型油菜杂交种亲本杂交种纯度SSR标记引物筛选
Brassica napusParentHybrid seed puritySSR markerPrimers selecting
《安徽农业科学》 2012 (11)
6376-6377,2
贵州省科研机构创新能力建设项目[黔科合院所(2009)004]贵州省农业科学院基金项目[黔农科合(基金)2010010].
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