安徽农业科学2012，Vol.40Issue(11)：6387-6388,6391,3.
聚电解质刷SiO2-g-PSStNa固定化果胶酶的研究
Study on Pectinase Immobilization Using SiO2-g-PSStNa Polyelectrolyte Brush
摘要
Abstract
[ Objective ] The aim was to study the immobilization methods of pectinase, to improve the activity of immobilized pectinase. [ Methods] Using the method of surface-initiated atom transfer radical polymerization( SI-ATRP) .nanometer composite material of SiO2-grafted PSStNa was composed based on SiO2 initiator. Moreover,the effect of immobilization on pH,temperature and storage stability of pectinase in aqueous phase medium was analyzed. [Result] SiO2-grafted PSStNa not only has core-shell structure,but also is "polyelectrolyte brush" with lots of negative charge enrich in the surface,pectinase was fixed on the "polyelectrolyte brush" .achieved the immobilization of pectinase with the characteristics of simple, mild and time-saving. The results showed that the optimum reaction pH and temperature of free and immobilized pectinase were 6.0 and 50℃.respectively. [Conclusion] The study results have great significance for the preparation and application of immobilized pectinase in the industry.关键词
表面原子转移自由基聚合/聚电解质/果胶酶/固定化Key words
Surface-initiated atom transfer radical polymerization/Polyelectrolyte/Pectinase/Immobilization分类
农业科技引用本文复制引用
毕淑娴,孙文娟..聚电解质刷SiO2-g-PSStNa固定化果胶酶的研究[J].安徽农业科学,2012,40(11):6387-6388,6391,3.基金项目
宁夏大学青年教师启动基金(QN200805). （QN200805）
