安徽农业科学2012，Vol.40Issue(11)：6401-6402,6473,3.
不同粗度灵武长枣休眠枝水培发芽情况及其无菌外植体的建立研究
Germination Status of Annual and Dormancy Branches of Chinese Jujube (Ziziphus jujuba Mill.) with Different Diameter by Water Culture Method and Their Aseptic Explants Establishment in Vitro
摘要
Abstract
[Objective] fhe aim was to study annual and dormancy branches of Chinese jujube with different diameter by water culture method,and their aseptic explants establishment. [ Method] Taking the annual and dormancy branches of Chinese jujube as the material to obtain aseptic ex-plants by water cultured method. [ Result] The water culture effect was good when the basic diameter of annual and dormancy branches of Chinese jujube was more than 3.5 cm,and the sprouting jujube head was more,healthy and strong. Taking this jujube head as explants,the survival rate increased to over 90% . The effects of surface sterilization was good with 75% ethanol 20 seconds + 0.1% mercuric chloride 15 min + twenn80 2 drops,and the pollution rate was 10.0%. [Conclusion] The study obtains the best scheme of accelerating germination of annual and dormancy branches of Chinese jujube by water method and buds sterilizing,which provides a scientific basis for prolonging the time of choosing materials and improving the sterile effects of buds,and provides a technological reference for researches on their tube breeding and striking roots and so on in future.关键词
灵武长枣/水培/休眠枝/无菌外植体Key words
Jujube( Ziziphus jujuba Mill. )/Water culture/Dormancy branches/Aseptic explants分类
农业科技引用本文复制引用
尚雁鸿,张存智,魏鹏..不同粗度灵武长枣休眠枝水培发芽情况及其无菌外植体的建立研究[J].安徽农业科学,2012,40(11):6401-6402,6473,3.基金项目
宁夏回族自治区科技攻关项目(宁夏优良品种优系快繁体系建立) （宁夏优良品种优系快繁体系建立）
宁夏职业技术学院校内课题(长枣优系微繁生根培养基研究). （长枣优系微繁生根培养基研究）
