安徽农业科学2012，Vol.40Issue(11)：6486-6487,6575,3.
缓冲液匀浆法和丙酮浸提法提取杨桃过氧化酶的研究
Study on the Extraction of Peroxidase in Carambola ( Averrhoa carambola) by Homogenate Buffer and Acetone Extraction
景艳艳 1杨昌鹏 1何新华2
作者信息
- 1. 广西大学农学院,广西南宁530004
- 2. 广西农业职业技术学院,广西南宁530007
- 折叠
摘要
Abstract
[Objective] The aim was to provide the theoretical basis for the separation and purification of peroxidase(POD) in carambola fruit. [Method] The POD in carambola was extracted using homogenate buffer and acetone extraction,and the extraction effects were compared. [Result ] The optimal conditions for star fruit POD by homogenate buffer were PVPP concentration of 1.0% , extraction time of 4 h, pH of 7.0; and the optimal conditions for star fruit POD by acetone extraction were acetone concentration of 90% .acetone extraction time of 30 min .solid-liquid radio of 1 : 4 g/ml. [Conclusion] The acetone extraction was better than homogenate buffer by comparing the two methods,which was about 15 times than the latter. Therefore, the extraction of peroxidase in carambola should select the acetone extraction method.关键词
杨桃/过氧化物酶/提取Key words
Carambola/Peroxidase/Extraction分类
农业科技引用本文复制引用
景艳艳,杨昌鹏,何新华..缓冲液匀浆法和丙酮浸提法提取杨桃过氧化酶的研究[J].安徽农业科学,2012,40(11):6486-6487,6575,3.
