安徽医科大学学报2012，Vol.47Issue(5)：492-495,4.
Mcl-1在阿霉素诱导骨肉瘤细胞凋亡中的作用
Effect of Mcl-1 on osteosarcoma cells under doxorubicin-induced apoptosis
摘要
Abstract
Objective To explore the expression level and functional role of Mcl -1 under doxorubicin induced ap- optosis. Methods The mRNA levels of Mcl4, Bcl-2 and β-actin were detected by semi-quantitative RT-PCR. The protein levels of Mcl-1, Bcl-2 and β-actin were detected by Western blot. Transfections of pcDNA-f1ag-Mcl-1 and pcDNA-f1ag -Bcl-2 were used to overexpress Mcl-1 and Bcl-2 in cells. Caspase-3/7 activities were measured by Caspase-3/7 assay kit. Results Although the expression of Mcl -1 and Bcl-2 mRNA stayed the same level, Mcl4 protein level decreased in osteosarcoma under doxorubicin induced apoptosis. Overexpression of Mcl-1 , not Bcl-2, significantly inhibited cleavage of PARP , which was marker for apoptosis. Meanwhile , overexpression of Mcl -1 also inhibited activity of caspase-3/7 by doxorubicin. Conclusion The downregulation of Mcl-1 protein level leads to an increase of apoptosis in osteosarcoma cells under doxorubicin treatment . In addition, this process is related to Caspase.关键词
骨肉瘤/Mcl-1/Bcl-2/阿霉素/细胞凋亡/CaspaseKey words
osteosarcoma/ Mcl-1 / Bcl-2/ doxorubicin/ cell apoptosis / Caspase分类
医药卫生引用本文复制引用
张耘,陈刚,戴守达,李群,张旭东,张林杰..Mcl-1在阿霉素诱导骨肉瘤细胞凋亡中的作用[J].安徽医科大学学报,2012,47(5):492-495,4.基金项目
安徽省教育厅自然科学基金(编号:KJ2011A167) （编号:KJ2011A167）
