安徽医科大学学报2012，Vol.47Issue(5)：495-499,5.
肝癌干细胞表达与血管生成拟态形成的研究
Research of the relationship between liver cancer stem cells and vasculogenic mimicry
摘要
Abstract
Objective To study the relationship between liver cancer stem cells and vasculogenic mimicry ( VM). Methods Three-dimensional cell cultures were established in several cell lines of HCC 97H, SMMC7721 and L02 cell, and observed the ability to form VM. Interesting cells which formed VM were isolated by the laser capture mi - crodissection, and endothelial related molecules of VE-cadherin, CD31 and liver cancer stem cells markers CD 133 and CD34 were analyzed by semi-quantitative reverse transcription -polymerase chain reaction (RT-PCR) and Western blot subsequently. Results HCC97H cell had the ability to form VM in three-dimensional cell cultures , while SMMC7721 and L02 cell hadn' t the ability. Interesting cells had been captured by LCM successfully. In comparative analysis, the expression level of VE-cadherin gene and protein in different groups were statistically significant (P <0. 05) , whereas CD31 was not expressed in the liver cancer cell lines . The mRNA and protein expressions of CD133 and CD34 in different groups were statistically significant ( P < 0. 05 ). Conclusion liver cancer stem cells may have impact on the formation of VM in hepatocellular carcinoma .n关键词
肝细胞癌/血管生成拟态/内皮相关分子/肿瘤干细胞Key words
hepatocellular carcinoma / vasculogenic mimicry/ endothelial related molecules / cancer stem cells分类
医药卫生引用本文复制引用
周腾,许戈良,刘文斌,张传海,荚卫东,任维华..肝癌干细胞表达与血管生成拟态形成的研究[J].安徽医科大学学报,2012,47(5):495-499,5.基金项目
国家自然科学基金面上项目(编号:30972892) （编号:30972892）
安徽省高校自然科学研究重点项目(编号:KJ2009A178) （编号:KJ2009A178）
安徽省自然科学基金面上项目(编号:090413107) （编号:090413107）
安徽省"115"产业创新团队-肝细胞癌转移复发研究团队资助项目 （）
