安徽医科大学学报2012，Vol.47Issue(5)：577-580,4.
临床分离表皮葡萄球菌的耐药性分析及与icaD基因表达关系的研究
Analysis of drug resistance and relationship with icaD genetypes of clinically isolated staphylococcus epidermidis
摘要
Abstract
Objective To detect the drug resistance of our ICU staphylococcus epidermidis to antibiotics, and to provide evidence for reasonable prevention and control measures to guide clinical medication . Methods mecA gene and icaD gene of the 125 strains staphylococcus epidermidis from clinical specimens and healthy skin were inspected and analyzed the drug resistance by K -B disk diffusion method and molecular biology method Results In two groups, icaD gene expression was correlated with mecA' s ( r = 0. 528, P = 0. 000; r = 0. 309, P = 0. 016 ) MecA gene expression in humor of clinical patients group was significantly higher than that in non -humor of normal people, and there was significant difference (P <0. 01) , while there was no significant difference in bacterial strain of icaD gene expression. Staphylococcus epidermidis in two groups were sensitive to rifampicin , nitrofurantoin, linezol-id and Vancomycin. The resistant rates of mecA gene expression bacterial strain to antibacterial agents had no sig -nificant difference in two groups , but the resistant rates of icaD gene expression had significant difference in ampi -cillin, cefoxitin, bactrim, erythromycin and chloramphenicol( P < 0. 05 ) Conclusion That ICU staphylococcus epidermidis to antibacterial agents have high drug resistance ,we must strengthen resistance monitoring, and according to antimicrobial susceptibility test results to choose rational drug , control nosocomial infection.关键词
表皮葡萄球菌/耐药性/PCR/mecA/icaDKey words
staphylococcus epidermidis/ drug resistance / PCR/ mecA/ icaD分类
医药卫生引用本文复制引用
曹晓光,周树生,戴媛媛,刘宝..临床分离表皮葡萄球菌的耐药性分析及与icaD基因表达关系的研究[J].安徽医科大学学报,2012,47(5):577-580,4.基金项目
安徽省教育厅课题基金资助项目(编号:KJ2009B001Z) （编号:KJ2009B001Z）
