安徽医科大学学报2012，Vol.47Issue(7)：753-757,5.
室旁核中活性氧在高血压大鼠交感神经活动过度增强中的作用
Effect of ROS in PVN mediate sympathetic overactivity in hypertension rats
摘要
Abstract
Objective To investigate the effect of reactive oxygen species ( ROS ) in paraventricular nucleus ( PVN ) mediated sympathetic overactivity in two-kindney, one-clip ( 2K1C ) hypertension rats. Methods Male SD rats were randomly divided into two groups, sham-operated group ( Sham ) and operation group ( 2K1C ), according to the technique of Goldblatt 2K1C established hypertension model. The conscious noninvasive method with tail cuff was performed in rats to record the systolic blood pressure ( SBP ). Mean arterial pressure ( MAP ), heat rate ( HR ) and renal sympathetic nerve activity ( RSNA ) were recorded in vivo on powerlab data-acquisiton systems. The paraventricular nucleus ( PVN ) was injected by brain stereotaxic instrument according to the Paxinos and Watson rat atlas. Results The SBP, MAP and RSNA were significantly enhanced in 2K1C compared to the Sham group. In both 2K1C and Sham groups, bilateral PVN microinjection of the superoxide anion scavenger 4-hydroxy-2,2,6,6-tetramethy-lpiperidinel-oxyl ( Tempol ) ( 0. 2 mol/L ) or NADPH oxidase inhibitor 4-hydroxy-3-methoxyacetophe-none ( APO ) ( 0. 01 mol/L ) and diphenyleneiodonium chloride ( DPI ) ( 0. 01 mol/L ) significantly decreased MAP and RSNA, but the MAP response of 2K1C was greater than that of Sham. Whereas, The SOD inhibitor diethyldi-thiocarbamic acid silver salt ( DETC ) ( 0. 1 mol/L ) could augment MAP and RSNA in both groups, but the MAP was significantly higher in 2K1C than that of Sham. Conclusion The data suggest that ROS in PVN takes part in sympathetic nerve overactivity and the development of 2K1C hypertension.关键词
室旁核/活性氧/高血压大鼠/肾交感神经活动/NADPH氧化酶Key words
paraventricular nucleus/ reactive oxygen/ hypertension rats/ renal sympathetic nerve activity/ NADPH oxidase分类
医药卫生引用本文复制引用
邹坤,李艳,王烈成,钟明奎..室旁核中活性氧在高血压大鼠交感神经活动过度增强中的作用[J].安徽医科大学学报,2012,47(7):753-757,5.基金项目
国家自然科学基金(编号:81070066) （编号:81070066）
安徽省教育厅自然科学重点项目(编号:KJ2010A176) （编号:KJ2010A176）
安徽医科大学博士科研基金(编号:XJ200823) （编号:XJ200823）
