安徽医科大学学报2012，Vol.47Issue(7)：758-762,5.
免疫调节肽对卵巢癌skov3细胞凋亡及相关基因表达的影响
Effect of immunomodulating peptide on ovarian cancer skov3 cell apoptosis and related gene expression
摘要
Abstract
Objective To study the effect of immunomodulatory peptide ( PGPIPN ) on human ovarian cancer skov3 cell apoptosis and related gene expression. Methods That skov3 cells cultured in vitro were incubated with different concentrations of PGPIPN ( 10 -6 ~ 10 -2 g/L ) for 48 h. The cDNA microarray technology was used to screen apoptosis related gene. Bax, Bcl-xl, Caspase-3 mRNA expression levels were detected by real-time fluorescent quantitative polymerase chain rection ( PCR ) method. Bax, Bcl-xl, Caspase-3 protein expression levels were detected by Western blot. Results Compared with the control group, the expressions of Bax and Caspase-3 were increased with the increase of the concentration of PGPIPN ( P < 0. 05 ). The expression of Bcl-xl was decreased with the increase of the concentration of PGPIPN ( P < 0. 05 ). Conclusion PGPIPN can induce the cell apoptosis by increasing the expression of Bax and decreasing the expression of Bcl-xl, this process may be related with Caspase-activated.关键词
免疫调节肽/cDNA芯片/Bax/Bcl-xl/Caspase-3Key words
immunomodulating peptide/ cDNA chip/ Bax/ Bcl-xl/ caspase-3分类
医药卫生引用本文复制引用
郑欣,何晓光,张文晓,杨浩然,魏彩,顾芳,任明强,秦宜德..免疫调节肽对卵巢癌skov3细胞凋亡及相关基因表达的影响[J].安徽医科大学学报,2012,47(7):758-762,5.基金项目
国家自然科学基金(编号:30872992) （编号:30872992）
