安徽医科大学学报2012，Vol.47Issue(7)：776-780,5.
PLCL/纤维蛋白原静电纺丝膜片与兔骨髓间充质干细胞的体外生物相容性
In vitro biocompatibility between electrospun nanofibrous films of PLCL/fibrinogen and rabbit bone marrow mesenchymal stem cells
摘要
Abstract
Objective To investigate the biocompatibility between electrospun nanofibrous films of PLCL/fibrino-gen nanofibrous films and rabbit bone marrow mesenchymal stem cells ( BMSCs ) in vitro. Methods Electrospun nanofibrous films were fabricated from poly( L-lactide-co-s-caprolactone )( PLCL ), and characterized by scanning electron microscopy( SEM ) and water contact angle analyzer. Take the cells seeded on PLCL/fibrinogen nanofibrous films as experimental group, and seeded on polystyrene culture plate as control group. The adhesion and proliferation results of rabbit BMSCs in two groups were detected by MTT assay. Results The cells used in this assay had the characteristics of mesenchymal stem cells( MSCs ). The average diameter and water contact angle of PLCL/ fibrinogen nanofibrous was ( 305 ±87 ) nm and ( 70. 21 ±2. 13 )°, respectively. Compared with control group, rabbit BMSCs in experimental group were exhibited similar adhesion and proliferation. Conclusion Electrospun PLCL/fibrinogen nanofibrous films have good performance in biocompatibility aspect and great potential applications in vascular tissue engineering field.关键词
静电纺丝/纳米纤维/组织工程/人工血管/间充质干细胞Key words
electrospinning/ nanofibrous/ tissue engineering/ artificial vascular/ mesenchymal stem cells分类
医药卫生引用本文复制引用
金良,胡何节,方征东..PLCL/纤维蛋白原静电纺丝膜片与兔骨髓间充质干细胞的体外生物相容性[J].安徽医科大学学报,2012,47(7):776-780,5.基金项目
安徽省自然科学基金(编号:11040606M199) （编号:11040606M199）
