高效液相色谱法测定山核桃外蒲壳中4,8-二羟基-1-四氢萘酮的含量 OACSTPCD
Determination of 4,8-Dihydroxy-1-tetralone in the Hull of Carya Cathayensis Sarg by High Performance Liquid Chromatography
目的 采用高效液相色谱法(high performance liquid chromatography,HPLC)测定山核桃外蒲壳中4,8-二羟基-1-四氢萘酮的含量.方法 采用HPLC,色谱柱为Shim-pack C18 (250 mm×4.6 mm,5 μm),以甲醇-水(40∶60)为流动相,流速为0.8 ml/min,检测波长为333 nm,柱温为25 ℃.结果 4,8-二羟基-1-四氢萘酮在1.13～20.34 μg/ml范围内与峰面积…查看全部>>
Objective To establish a high performance liquid chromatography (HPLC )method for determining the contents of 4,8-Dihydroxy-1-tetralone in the hull of Carya cathayensis Sarg. Methods The HPLC determination was carried out on a Shim -pack C18 (250 mm × 416 mm , 5 μm ) column with mobile phase of methanol-water (40:60 ) , flow rate of 0 .8 ml/min , column temperature of 25 ℃ , and detection wavelength of 333 nm . Results The concentration of 4 ,8-di…查看全部>>
欧金梅;陈仕云;吴德玲
安徽中医学院药学院,现代中药安徽省重点实验室,安徽,合肥,230031安徽中医学院药学院,现代中药安徽省重点实验室,安徽,合肥,230031安徽中医学院药学院,现代中药安徽省重点实验室,安徽,合肥,230031
药学
山核桃外蒲壳48-二羟基-1-四氢萘酮高效液相色谱法
hull of Carya cathayensis Sarg 4 ,8-Dihydroxy-1-tetralone high performance liquid chroma tography
《安徽中医学院学报》 2012 (3)
66-68,3
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