安徽中医学院学报2012，Vol.31Issue(3)：69-71,3.DOI:10.3969/j.issn.1000-2219.2012.03.024
藤黄中新藤黄酸提取分离工艺改进
Improvement of Extraction and Isolation Technology for Neogambogic Acid from Gamboge
摘要
Abstract
Objective To improve the extraction and isolation technology for neogambogic acid from Gamboge .Methods The extracting process was optimized by orthogonal design . Alcohol was used as extraction solvent ,AB-8 macroporous resin was employed to enrich extracts , silica gel G was used as stationary phase in wet chromatography column with a mobile phase consisted of petroleum ether -alcohol (20:1) .Finally ,a golden yellow crystal compound was obtained . The structure of the obtained compound was identified by melting point (mp) determination, ultraviolet spectra (UV ), infrared spectra (IR), mass spectra (mass spectra ,MS ) 1H-nuclear magnetic resonance (NMR) ,and 13C-NMR . Results The data of mp ,UV ,IR ,MS ,1H-NMR, and 13C-NMR for the obtained golden yellow crystal compound were in accordance with those recorded in related literatures , correspondingly .Conclusion The improved technology is feasible for the extraction and isolation of neogambogic acid from Gamboge ,and is beneficial to industrialization production .关键词
藤黄/新藤黄酸/提取/分离/工艺改进Key words
Gamboge / neogambogic acid / extraction / isolation / technology improvement分类
中医学引用本文复制引用
刘修树,周娟,王效山..藤黄中新藤黄酸提取分离工艺改进[J].安徽中医学院学报,2012,31(3):69-71,3.基金项目
安徽省教育厅质量工程特色专业项目(皖教高[2010]28号) （皖教高[2010]28号）
巢湖职业技术学院科研项目(zrkx200804) （zrkx200804）
