北京科技大学学报2012，Vol.34Issue(5)：602-607,6.
乳酸菌USTB--08的高效培养和生产乳酸
High-density cultivation of Lactobacillus sp. USTB-08 and production of lactic acid
摘要
Abstract
Based on the successful isolation of a bacterial strain of Lactobacillus sp. USTB-08 for producing lactic acid, the opti- mal control conditions including carbon source, nitrogen source, mass ratio between carbon and nitrogen source, temperature and pH values were investigated for the growth of Lactobacillus sp. USTB-08 and the production of lactic acid in batch cultivation and fed-batch fermentation. Sucrose as the sole carbon source, yeast extract as the sole nitrogen source, the mass ratio of carbon to nitrogen source of 5： 1, 35℃, the inoculum amount of 1.0% and initial pH 6. 50 are the optimal parameters for the growth of Lactobacillus sp. USTB-08 in batch cultivation. In fed-batch cultivation by feeding a mixture of 10% sodium hydroxide and 25% sucrose to control the pH value and supply the carbon source in a 50 L automatic fermenter, the maximum biomass of Lactobacillus sp. USTB-08 （OD680nm 13.2） and the maximum content of lactic acid （28.0g-L-1） are obtained at pH 6. 50 and pH 7.00 in a pH range from 6.00 to 7. Off, respective- ly. The strategy of feeding sucrose and controlling the pH value simultaneously was firstly used in the fed-batch cultivation of Lactoba-cillus sp. , and both high cell density and high lactic acid content were obtained.关键词
乳酸菌/培养/生长/乳酸/pH控制Key words
LactobaciUus sp./cultivation/growth/lactic acid/pH control分类
生物工程引用本文复制引用
吕乐,张可毅,赵鹏,尹春华,刘晓璐,许倩倩,闫海..乳酸菌USTB--08的高效培养和生产乳酸[J].北京科技大学学报,2012,34(5):602-607,6.基金项目
国家自然科学基金资助项目 （）
中央高校基本科研业务费专项资金资助项目 （）
